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Vaccine Detail
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PvRII/ AS02A |
| Vaccine Information |
- Vaccine Name: PvRII/ AS02A
- Target Pathogen: Plasmodium spp.
- Target Disease: Malaria
- Type: Subunit vaccine
- Status: Research
- Host Species for Licensed Use: Macaque
- Antigen: PvRII, receptor-binding domain of Plasmodium vivax Duffy binding protein, region II, with a C-terminal 6-histidine tag expressed in E. coli. (Moreno et al., 2008)
- PvDBPII
gene engineering:
- Type: Recombinant protein preparation
- Description: The designed gene encoding PvRII was synthesized using overlapping oligomers (Midland Certified Reagent Company) and cloned in plasmid pET28a(+) (Novagen) at NcoI and SalI sites. Expression of recombinant PvRII using synthetic gene was higher compared with native gene. (Yazdani et al., 2006)
- Detailed Gene Information: Click Here.
- Preparation: PvRII from P. vivax Salvador I strain was cloned as a NcoI-SalI fragment in the E. coli expression vector pET28a(+). Protein expression of the recombinant 6-His tag PvRII was induced with 1 mM IPTG for 4 hours and purified. 50 μg and 10 μg of PvRII formulated in AS02A. (Moreno et al., 2008)
- Immunization Route: Intramuscular injection (i.m.)
- Description: The PvRII/ AS02A vaccine uses recombinant PvRII (region II), the receptor-binding domain of the Plasmodium vivax Duffy binding protein. Antibodies raised against the P.vivax Duffy binding protein, which belong to a family of erythrocyte binding protiens residing in region II, block erythrocyte invasion in the malaria infection process. Formulations emulsified with the AS02A adjuvant elicted higher titer binding inhibitory antibodies compared to other adjuvants such as Alhydrogel and MF59. (Moreno et al., 2008)
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| Host Response |
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| References |
Moreno et al., 2008: Moreno A, Caro-Aguilar I, Yazdani SS, Shakri AR, Lapp S, Strobert E, McClure H, Chitnis CE, Galinski MR. Preclinical assessment of the receptor-binding domain of Plasmodium vivax Duffy-binding protein as a vaccine candidate in rhesus macaques. Vaccine. 2008; 26(34); 4338-4344. [PubMed: 18573299].
Yazdani et al., 2006: Yazdani SS, Shakri AR, Pattnaik P, Rizvi MM, Chitnis CE. Improvement in yield and purity of a recombinant malaria vaccine candidate based on the receptor-binding domain of Plasmodium vivax Duffy binding protein by codon optimization. Biotechnology letters. 2006; 28(14); 1109-1114. [PubMed: 16794771].
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