|
|
Vaccine Detail
|
Klebesiella FuyA protein vaccine |
| Vaccine Information |
- Vaccine Name: Klebesiella FuyA protein vaccine
- Target Pathogen: Klebsiella pneumoniae
- Target Disease: Pneumonia, Bloodstream infections, Abscess
- Type: Subunit vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- Antigen: FuyA (Yersinia bactin receptor FyuA) (Kumar et al., 2020)
- fyuA
gene engineering:
- Type: Recombinant protein preparation
- Description: Yersiniabactin receptor coding gene FyuA of Klebsiella pneumoniae ATCC 43816 was amplified by colony PCR using the primers Forward 5′- AAGGATCCATGAAAATGACACGGCTTTAT-3'and Reverse5′- AACTCGAGTCAGAAGAAATCAATTCGCGT-3′ which were designed for the sequence with accession id NC_012731.1. The restriction sites BamHI and XhoI were present in forward and reverse primers respectively to generate sticky ends for cloning into the vector. The amplified gene was cloned into pET28a to produce N-terminally tagged hexahistidine fusions. The resulting constructs were verified by sequencing. The constructs without mutations were transformed into E.coli BL21 expression host. The expression was induced by the addition of 1 mM IPTG and further incubation at 37 °C with aeration for 4 h before harvesting the cells by centrifugation (Kumar et al., 2020).
- Detailed Gene Information: Click Here.
- Allergen: FuyA (Yersinia bactin receptor FyuA)
- Preparation: Yersiniabactin receptor coding gene FyuA of Klebsiella pneumoniae ,ATCC 43816 was amplified by colony PCR.The amplified gene was cloned into pET28a to produce N-terminally tagged hexahistidine fusions (Kumar et al., 2020)
- Immunization Route: intranasal immunization
|
| Host Response |
|
|
| References |
Kumar et al., 2020: Kumar A, Harjai K, Chhibber S. Early cytokine response to lethal challenge of Klebsiella pneumoniae averted the prognosis of pneumonia in FyuA immunized mice. Microbial pathogenesis. 2020; 144; 104161. [PubMed: 32194179].
|
|
