• Vaccine Name: RPV ISCOM Vaccine
• Target Pathogen: rinderpest virus
• Type: Subunit vaccine
• Status: Research
• Host Species for Licensed Use: None
• Antigen: H protein (Kamata et al., 2001)
• H protein gene engineering:
  • Type: Recombinant protein preparation
  • Description: (Kamata et al., 2001)
  • Detailed Gene Information: Click Here.
• Preparation: The recombinant baculovirus expressed the RPV H protein as a membrane-bound protein in infected Sf21 insect cells and the protein was purified by solubilizing purified cell membranes with octylglycoside. ISCOMs incorporating the RPV H protein were produced according to a standard method. (Kamata et al., 2001)
• Immunization Route: subcutaneous injection
• Description: Rinderpest virus (RPV) ISCOM (Immune-stimulating complex) vaccine induces protection in cattle against virulent RPV challenge. (Kamata et al., 2001)

Cattle Response

• Vaccination Protocol: Four Friesian cross Aberdeen Angus calves were inoculated subcutaneously with the ISCOM vaccine containing 100 mg RPV H protein in a volume of 1.0 ml. After 5 or 6 weeks, the cattle received the second vaccination with the ISCOM vaccine incorporating 50 mg RPV H protein. Three cattle were used as unvaccinated controls. (Kamata et al., 2001)
• Immune Response: Two of the four cattle developed significant levels of neutralizing antibody after the first vaccination, while after the second vaccination, high levels of neutralizing antibody were present in all four animals. Following challenge, a slight increase in antibody titers was observed in the completely protected cattle, whereas a rapid rise in antibody titer was seen in the partially protected animal. The control cattle remained antibody-negative throughout the experiment. (Kamata et al., 2001)
• Challenge Protocol: All seven animals were challenged with 104TCID50 of virulent Saudi 1/81 strain of RPV at 25 weeks or 15 weeks after the first vaccination. (Kamata et al., 2001)
• Efficacy: The three unvaccinated controls developed severe clinical signs of rinderpest, high fever, severe stomatitis and diarrhea, and were euthanized on either day 9 or 10 following challenge. In contrast, all four vaccinated cattle survived the chal- lenge. Three of the four vaccinated cattle were solidly protected from the disease and showed no clinical signs of infection throughout the experiment. The remaining animal developed a delayed and transient fever and a mild mouth erosion but quickly recovered. (Kamata et al., 2001)