• Vaccine Name: IPNV Inactivated Vaccine GS2019-4
• Target Pathogen: Infectious pancreatic necrosis virus
• Type: Inactivated or "killed" vaccine
• Status: Research
• Host Species for Licensed Use: None
• Antigen: Genogroup I IPNV (Duan et al., 2022)
• Preparation: Chinook salmon embryo cells (CHSE-214) were cultured in Minimal Essential Medium (MEM, Gibco, Cat No: C11095500BT) including 10% fetal bovine serum (FBS, Gibco, Cat No: 10099141) at 18 °C, and was used to amplify IPNV at 15 °C in vitro in this study. (Duan et al., 2022)
• Immunization Route: Intraperitoneal injection (i.p.)
• Description: IPNV isolate GS2019-4 inactivated vaccine could induce a non-specific and specific immune response in rainbow trout, which are responsible for the anti-IPNV ability at an early and late stage after immunization. (Duan et al., 2022)

Fish Response

• Vaccination Protocol: Rainbow trout (n = 30) were intraperitoneally injected with the inactivated virus with a dose of 200 μl per fish (2.50 × 10^5.0 PFU). The injected fish were maintained in the circulating aquarium (15 °C), fed a commercial pelleted diet, and the healthy condition was observed for 30 days. (Duan et al., 2022)
• Immune Response: The viral loads ratio (PBS group/immunization group) was calculated to present the antivirus effect of the IPN vaccine. The higher the ratio is, the stronger the antiviral effect is. Viral loads in immunized rainbow trout at each time point were significantly lower than that in the PBS group (Table 2), indicating that the vaccination function in resistance to virus infection. The strongest antiviral effect was observed on 30 d.p.i, the viral loads ratio at this time point was significantly higher than those on other time points (p < 0.05), and the mean viral loads ratio at this time point was 1.00 × 10^4.73 (53703.17) folds. The weakest antiviral effect was observed on 3 d.p.i, and the viral loads ratio was 1.0 × 100.66 (4.57 folds), but the viral loads in the immunized group were still significantly lower than that in the negative control group at this time point (p < 0.05). (Duan et al., 2022)
• Challenge Protocol: Rainbow trout (n = 60) were intraperitoneally injected with IPNV at the dose of 10 μl per fish (1.25 × 10^4.0 PFU), and the rainbow trout injected with phosphate buffer solution (PBS) were used as the negative control. (Duan et al., 2022)
• Efficacy: The viral loads in the immunized rainbow trout were significantly decreased, indicating that the vaccine had good immune protection. (Duan et al., 2022)