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Vaccine Detail
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Bovine Leukemia Virus Vaccine pBLV6073DX |
| Vaccine Information |
- Vaccine Name: Bovine Leukemia Virus Vaccine pBLV6073DX
- Target Pathogen: Bovine Leukemia virus
- Type: Recombinant vector vaccine
- Status: Licensed
- Host Species for Licensed Use: Cattle
- Antigen: Vaccine strain (pBLV6073DX) with a point mutation in the transmembrane protein gene (T>G at nucleotide 6073) and a partial deletion of the R3-G4 sequences (between positions 6614 and 6997). (Suárez et al., 2022)
- Preparation: The proviral construct inserted in the pSP64 vector (Promega) was amplified in Escherichia coli C3040 (New England Biolabs). The standard protocol for production of the inoculum was based on transfection of a monolayer of CHOK1 cells in 25 cm2 cell culture flasks with 2.6 µg of pBLV6073DX and 5.2 µg of linear polyethylenimine (Polysciences) in the presence of 3 ml EMEM (Sigma-Aldrich). Four hours post-transfection, 3 ml of EMEM supplemented with 10% FBS (Internegocios SA) and 3% trehalose were added. Seventy-two hours post-transfection, lysate aliquots (6 ml/dose) were preserved at -80°C in the presence of 15% trehalose. (Suárez et al., 2022)
- Immunization Route: subcutaneous injection
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