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Vaccine Detail
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Crimean-Congo Haemorrhagic Fever (CCHF) Virus Vaccine CCvax |
| Vaccine Information |
- Vaccine Name: Crimean-Congo Haemorrhagic Fever (CCHF) Virus Vaccine CCvax
- Target Pathogen: Crimean-Congo Haemorrhagic Fever (CCHF)
- Type: Inactivated or "killed" vaccine
- Status: Research
- Host Species for Licensed Use: None
- Antigen: Turkey-Kelkit06 Virus
- Preparation: To produce CCVax antigens, Vero E6 cell monolayers in 175 cm2 flasks (Corning, NY, USA, 431079) were inoculated with 0.01 MOI of Turkey-Kelkit06 CCHFV after the assessment of virus growth in MOI optimization studies. The virus was diluted in 5 ml of DMEM F-12 (w/o serum), and the cells were covered with the virus inoculum. The flasks were left at 37 °C for 60 min and hand-rotated every 15 min during incubation. The inocula were discarded, and the cells were washed twice with PBS; then, the flasks were filled with 20 ml of virus growth medium (2% FBS in DMEM F-12) containing 1% antibiotic (Pen/Strep/Amph) (Sigma-Aldrich, Germany, A5955). The flasks were left at 37 °C in a constant 5% CO2-supplemented humidified cell-culture chamber until cells showing lysis began to detach from the bottom of the flask. To produce the MBVax, 1 × 104 PPFU of Turkey-Kelkit06 was administered to 3-day-old suckling BALB/c mice intracerebrally through the forehead. The mice were left in their home cages with their mothers. Mice were euthanized when they started to show partial limb paralysis (hind limb paralysis was seen between approx. 4 and 5 days after inoculation). The brains were harvested from the paralytic infected mice, minced into several pieces and equally (w/v) mixed with the virus growth medium. The brain samples were freeze–thawed twice, subjected to bench-top cooled centrifugation at 12,000 rpm for 30 min to remove brain debris, and stored at −80 °C in a freezer in aliquots. (Berber et al., 2021)
- Immunization Route: Other
- Description: An cell-culture-derived inactivated CCHF vaccine mixed with aluminum adjuvant.
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