• Vaccine Name: T. cruzi vaccine encoding Tc80
• Target Pathogen: Trypanosoma cruzi
• Target Disease: Chagas disease
• Type: DNA vaccine
• Status: Research
• Host Species for Licensed Use: Mouse
• Antigen: Tc80 (Bivona et al., 2018)
• Tc80 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001237
• Immunization Route: Intramuscular injection (i.m.)
• Description: T. cruzi sub-unit vaccine formulated with recombinant Tc80 plus oligodeoxynucleotides CpG (ODN-CpG) as adjuvant. (Bivona et al., 2018)

Mouse Response

• Host Strain: C3H/HeN
• Host age: 7 - week old
• Vaccination Protocol: Mice were immunized with four doses separated by ten days intramuscularly in the quadriceps with 10ug of rTc80 adjuvanted with 10 ug of ODN-CpG 1826. Control group mice were intramuscularly injected twice with PBS + 10 ug CpG-ODN and then two does of attenuated Salmonella carrying an empty plasmid pcDNA 3.1 orally. (Bivona et al., 2018)
• Immune Response: Mice elicited antibody titers considerably higher than control group. Splenocyts from all immunized mice were able to secrete IL-2 and IFN-y upon antigen recall. Mice presented a higher percentage of IFN-y and TNF-a producing CD4+ T cells compared with control group. (Bivona et al., 2018)
• Challenge Protocol: Two weeks after the last dose of vaccination protocols, immunized male C3H/HeN mice were challenged intraperitoneally with 2.5x105 blood trypomastigotes of the sub-lethal T. cruzi strain K98. (Bivona et al., 2018)
• Efficacy: All immunized mice presented significantly reduced parasitemias during the acute phase of infection compared to the control. rTc80im group achieved the highest control of parasitemia. (Bivona et al., 2018)