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Vaccine Detail
T. cruzi Tc24 vaccine |
Vaccine Information |
- Vaccine Name: T. cruzi Tc24 vaccine
- Target Pathogen: Trypanosoma cruzi
- Target Disease: Chagas disease
- Type: Subunit vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- Antigen: Tc24 (Barry et al., 2019) - Tc24 has been found to induce strong CD8+ T-cell responses in past studies and has shown promise to be effective against a wide diversity of T. cruzi parasite strains (Arnal et al., 2020)
- Tc24 (Obselete)
gene engineering:
- Type: Recombinant protein preparation
- Description:
- Detailed Gene Information: Click Here.
- Immunization Route: Intramuscular injection (i.m.)
- Description: Vaccine containing recombinant protein Tc24 formulated with an emulsion containing the Toll-like receptor 4 against E6020 to combat chronic T. cruzi infection. (Barry et al., 2019)
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Host Response |
Mouse Response
- Host Strain: BALB/c
- Vaccination Protocol: Mice were infected with T. cruzi and allowed to progress past the acute phase of disease, characterized by elevated parasitemia resolving by 40 days post-infection, before vaccination at 70 days post-infection with Tc24+E6020-SE or a sham vaccine. (Barry et al., 2019)
- Immune Response: The vaccine elicits a greater than 5-fold increase in Tc24-specific secreted IFNγ compared to the Tc24 control. IgG2a, a mouse antibody isotype associated with a TH1-bias, is most robust in the vaccine compared to the controls, further validating the cytokine results. In comparison, both the Tc24+E6020-SE vaccine and the Tc24 control produce a robust IgG1 antibody response. These results indicate that the Tc24 recombinant protein is capable of producing an antigen-specific immune response, but that the E6020-SE adjuvant is necessary to induce a favorable TH1-biasing of the resulting immune response. (Barry et al., 2019)
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References |
Arnal et al., 2020: Arnal A, Villanueva-Lizama L, Teh-Poot C, Herrera C, Dumonteil E. Extent of polymorphism and selection pressure on the Trypanosoma cruzi vaccine candidate antigen Tc24. Evolutionary applications. 2020; 13(10); 2663-2672. [PubMed: 33294015].
Barry et al., 2019: Barry MA, Versteeg L, Wang Q, Pollet J, Zhan B, Gusovsky F, Bottazzi ME, Hotez PJ, Jones KM. A therapeutic vaccine prototype induces protective immunity and reduces cardiac fibrosis in a mouse model of chronic Trypanosoma cruzi infection. PLoS neglected tropical diseases. 2019; 13(5); e0007413. [PubMed: 31145733].
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