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Vaccine Detail
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Duck Enteritis Virus Vaccine rDEV-UL26/27-P13C |
| Vaccine Information |
- Vaccine Name: Duck Enteritis Virus Vaccine rDEV-UL26/27-P13C
- Target Pathogen: Duck enteritis virus
- Target Disease: Duck Plague
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: None
- Host Species as Laboratory Animal Model: Duck
- Antigen: (Yang et al., 2021) whole virus
- Preparation: (Yang et al., 2021)The P1-P2A-3C cassette was inserted into the gene junction between UL26 and UL27. in the DEV vaccine strain genome. For transfection, we used the modified fosmids, C144-UL26/27-P13C, which replaced the parental fosmid C144. After being blindly passaged once in CEFs, DEV-typical plaques appeared in the CEFs transfected with the DNA combinations (Figure 2A). Electron microscopy confirmed the successful rescue of the recombinant viruses. Insertion of the P1-P2A-3C cassette at the proper site was confirmed by PCR and sequencing, using a forward primer specific to the P1 gene and a reverse primer matching the UL26 sequences. The recombinant DEV was designated rDEV-UL26/27-P13C, with the P1 and 3C genes inserted between UL26 and UL27 in the DEV genome.
- Immunization Route: Intramuscular injection (i.m.)
- Description: A bivalent duck enteritis virus recombinant vector vaccine that is made of a P1-P2a-33 cassette inserted between UL26 and UL27 of the viral genome (Yang et al., 2021).
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