• Vaccine Name: SARS-CoV pCI-N DNA from vaccine
• Target Pathogen: SARS-CoV
• Target Disease: Severe Acute Respiratory Syndrome (SARS)
• Type: DNA vaccine
• Status: Research
• Host Species for Licensed Use: None
• Host Species as Laboratory Animal Model: mouse
• Antigen: S protein (Zhao et al., 2005)
• nucleocapsid protein (N) gene of SARS-CoV gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• Immunization Route: Intramuscular injection (i.m.)
• Description: A plasmid pCI-N, encoding the full-length N gene of SARS-CoV, was constructed and expressed in Escherichia coli DH5alpha (Zhao et al., 2005)

Mouse Response

• Host Strain: Balb/c(Zhao et al., 2005)
• Vaccination Protocol: 200 μg of Vaccine 573 in both tibialis anterior muscles three times at 2-week intervals (Zhao et al., 2005)
• Immune Response: Anti-N immunoglobulins (specifically IgG2a) and splenocytes proliferative responses against N protein, splenocyte production of IFN-γ, IL-2, IL-4, IL-10, production of N-specific CD8+ T cells, delayed-type hypersensitivity response
  (Zhao et al., 2005)
• Side Effects: Delayed hypersensitivity response (Zhao et al., 2005)