• Vaccine Name: Recombinant spike polypeptide vaccine
• Target Pathogen: SARS-CoV
• Target Disease: Severe Acute Respiratory Syndrome (SARS)
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: None
• Host Species as Laboratory Animal Model: mouse
• Antigen: S protein (Woo et al., 2005)
• S protein gene of SARS-CoV gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Immunization Route: Intraperitoneal injection (i.p.)
• Description: intraperitoneal recombinant spike polypeptide generated by amplify gene encoding amino acids residues 14-667 of S protein that was cloned BamHI and KpnI sites of vector pQE-31 that was generated by Escherichia coli (Woo et al., 2005)

Mouse Response

• Host Strain: Balb/c (H-2d)
• Host age: 6-8 weeks (Woo et al., 2005)
• Host gender: Male (Woo et al., 2005)
• Vaccination Protocol: 50 μg administered via intraperitoneal route on days 0, 14, and 28. (Woo et al., 2005)
• Immune Response: No neutralizing antibody production, high IgG levels, lymphocyte proliferation, production of IFN-γ >6000 pg/ml at 48hrs and 72hrs, detectable production of IL-4 at 24hrs (Woo et al., 2005)