• Vaccine Name: MERS England1 S DNA + MERS England1 S protein subunit vaccine
• Target Pathogen: MERS-CoV
• Target Disease: Middle East Respiratory Syndrome (MERS)
• Type: Mixed, DNA vaccine and Subunit vaccine
• Status: Research
• Host Species for Licensed Use: None
• Host Species as Laboratory Animal Model: mouse, rhesus monkey
• Immunization Route: Intramuscular injection (i.m.)
• Description: A mixed vaccine using England S1 subunit protein vaccine and England1 S DNA vaccine. VRC8400 plasmid used to construct antigen (Wang et al., 2015)

Mouse Response

• Host Strain: BALB/cJ
• Host age: 6-8 weeks (Wang et al., 2015)
• Host gender: Female (Wang et al., 2015)
• Vaccination Protocol: S1 MERS-CoV full-length Spike protein at weeks and 3 and then injected with S1 protein plus Ribi adjuvant (Sigma-Aldrich, St. Louis, MO) at week 6 (Wang et al., 2015)
• Immune Response: high titers of neutralizing antibody (Wang et al., 2015)
• Challenge Protocol: Animals were challenged with JordanN3 strain of MERS-CoV (Wang et al., 2015)

Macaque Response

• Host Strain: Macaca mulatta (Wang et al., 2015)
• Host gender: Male and Female (Wang et al., 2015)
• Vaccination Protocol: Six NHPs in the S DNA-S1 protein group were injected with 1 mg of plasmid DNA encoding MERS-CoV full-length Spike at weeks 0 and 4 and boosted with 100 μg of MERS-CoV S1 protein and AlPO4 adjuvant (Brenntag Biosector, Frederikssund, Denmark) at week 8 (Wang et al., 2015)
• Challenge Protocol: challenged with Jordan N3 strain of MERS-CoV 19 weeks after immunization at 3.1e6, 3.6e6, and 3.4 e6 p.f.u. (Wang et al., 2015)
• Efficacy: protected (Wang et al., 2015)