• Vaccine Name: rS.C-APP-ApxIIA
• Target Pathogen: Actinobacillus pleuropneumoniae
• Target Disease: porcine pleuropneumonia
• Vaccine Ontology ID: VO_0004786
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• apxIIA gene engineering:
  • Type: Recombinant vector construction
  • Description: A neutralizing epitope fragment of ApxIIA toxin was expressed on the cell surface of Saccharomyces cerevisiae (Kim et al., 2010).
  • Detailed Gene Information: Click Here.
• Vector:
  • Vector name:
  • VO vector ID: VO_0001122
  • Description: (Kim et al., 2010)
• Preparation: (Kim et al., 2010) A neutralizing epitope fragment of ApxIIA toxin was expressed and immobilized on the cell surface of Saccharomyces cerevisiae for efficient vaccine development.
• Immunization Route: Oral vaccination

Mouse Response

• Vaccination Protocol: Oral immunization was preceded by overnight fasting of the mice (water was provided ad libitum). Freshly harvested 2.5 x 10^7 or 2.5 x 10^8 cells were dissolved into 1ml of 0.9% saline and orally administered at 200 µl/mouse through an oral gavage at 10 day intervals, 4 times (Kim et al., 2010).
• Vaccine Immune Response Type: VO_0003057
• Challenge Protocol: The immunized mice were injected intraperitoneally with 200 ml of an A. pleuropneumoniae preparation (about 1 x 10^8 CFU) or buffer control 10 days after the final immunization (Kim et al., 2010).
• Efficacy: The mice fed the recombinant epitope-expressing yeast were protected from injection of a lethal dose of A. pleuropneumoniae (Kim et al., 2010).