• Vaccine Name: rVEE-BoNT/A
• Target Pathogen: botulinum neurotoxin
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• Vector:
  • Vector name:
  • VO vector ID: VO_0001124
  • Description: (Lee et al., 2001)
• Preparation: The vaccine vector is composed of a self-replicating RNA containing all of the VEE nonstructural genes and cis-acting elements and also a heterologous immunogen gene placed downstream of the subgenomic 26S promoter in place of the viral structural genes (Lee et al., 2001).
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: Mice were vaccinated with various doses of H(C)-VRP at different intervals (Lee et al., 2001).
• Vaccine Immune Response Type: VO_0000287
• Challenge Protocol: Mice were intraperitoneally challenged with up to 100,000 50% lethal dose units if BoNT/A (Lee et al., 2001).
• Efficacy: The mice exhibited protection from the challenge, however protection correlated directly with serum enzyme-linked immunosorbent assay titers to BoNT/A. The duration of the immunity achieved was tested at 6 months and at 1 year postvaccination, and mice challenged at these times remained refractory to challenge with BoNT/A (Lee et al., 2001).