• Vaccine Name: rPIV5-H3 (influenza virus)
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004783
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• Vector:
  • Vector name:
  • VO vector ID: VO_0001112
  • Description: The bovine parainfluenza virus vaccine vector can be used to express other pathogen's gene (Tompkins et al., 2007).
• Preparation: DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses (Tompkins et al., 2007).
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: Mice were given an intraperitoneal injection of 40 μg peptide-KLH or unconjugated KLH in complete Freund’s adjuvant (emulsified 1:1 with antigen in PBS). Three weeks later, the mice were given an intraperitoneal booster injection with peptide-KLH in incomplete Freund’s adjuvant; 13 days later blood was collected (Tompkins et al., 2007).
• Vaccine Immune Response Type: VO_0000287
• Challenge Protocol: Challenge virus in 50 μL of PBS was administered intranasally to anesthetized mice. Isoflurane or ketamine/xylazine was used for mice challenged with H1N1 subtype. Reported 50% lethal dose (LD50) for H1N1 subtype was determined for 8-week-old naive BALB/c mice for each anesthetic (and may vary from the actual LD50 for the older vaccinated mice that were challenged). Subtype H5N1 was administered intranasally to mice anesthetized with 2,2,2-tribromoethanol in tert-amyl alcohol (Tompkins et al., 2007).
• Efficacy: ice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that crossreacted with human and avian M2 sequences and produced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain (Tompkins et al., 2007).