• Vaccine Name: vFP89-HA (H5N1 HPAI virus)
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004745
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• HA gene engineering:
  • Type: Recombinant protein preparation
  • Description: Fowlpox (FP) vector vaccines expressing the native hemagglutinin (HA) gene from H5N8 (Bublot et al., 2010).
  • Detailed Gene Information: Click Here.
• Vector:
  • Vector name:
  • VO vector ID: VO_0001040
• Preparation: Compare one (vFP89) expressing the native hemagglutinin (HA) gene from H5N8 A/turkey/ Ireland/1378/83 and the other (vFP2211) expressing a modified synthetic HA gene from H5N1 A/chicken/Indonesia/7/2003 (Bublot et al., 2010).
• Immunization Route: Intramuscular injection (i.m.)

Chicken Response

• Vaccination Protocol: Four groups of 20 1-day-old specific-pathogen-free chickens were made: Groups 1 and 2 were immunized with 3 log10 tissue-culture infectious dose 50% (TCID50) of vFP89 and vFP2211, respectively, whereas group 3 was immunized with vFP89, but received a booster immunization at 2 wk of age with an inactivated vaccine containing A/turkey/Wisconsin/68 H5N9 virus (inH5N9); group 4 was left unvaccinated (Bublot et al., 2010).
• Vaccine Immune Response Type: VO_0003057
• Challenge Protocol: Ten birds from each group were challenged on day 21 with A/turkey/Turkey/1/2005 clade 2.2 H5N1 HPAI virus (Bublot et al., 2010).
• Efficacy: All directly challenged and 9/10 nonvaccinated contact chickens died after challenge (mean death time of 2.3 and 6.1 days, respectively) and most of them shed virus before death via cloacal and buccal routes. All vaccinated birds were clinically protected from HPAI challenge. One (vFP2211), 2 (vFP89+inact.), or 3 (vFP89) out of the 10 directly challenged vaccinated chickens shed virus via the buccal route 2-5 days postinfection (Bublot et al., 2010).