• Vaccine Name: CVD 103-HgR- REPEC
• Target Pathogen: Escherichia coli
• Target Disease: Hemorrhagic colitis
• Vaccine Ontology ID: VO_0004721
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• Vector:
  • Vector name:
  • VO vector ID: VO_0001044
• Preparation: Rabbit Enteropathogenic E. coli (REPEC), a member of the AEEC family. The C-terminal portion of intimin was delivered by the attenuated Vibrio cholerae vaccine strain CVD 103-HgR. To export intimin, a fusion was engineered with ClyA, a secreted protein from Salmonella enterica serovar Typhi (Keller et al., 2010).
• Immunization Route: Intramuscular injection (i.m.)

Rabbit Response

• Vaccination Protocol: Three groups of six rabbits were primed on day 1 with one of three inocula: PBS, CVD 103-HgR (pSEC 91), or CVD 103-HgR (pInt248) and boosted on day 15 with the identical inoculum (Keller et al., 2010).
• Vaccine Immune Response Type: VO_0003057
• Challenge Protocol: Rabbits were challenged on day 29 with wild type REPEC strain E22 (6 × 10^7 CFU) (Keller et al., 2010).
• Efficacy: After immunization, antibodies specific to intimin from serum and bile samples were detected and moderate protection against challenge with a virulent REPEC strain was observed. Compared to animals immunized with vector alone, intimin-immunized rabbits exhibited reduced fecal bacterial shedding, milder diarrheal symptoms, lower weight loss, and reduced colonization of REPEC in the cecum. V. cholerae CVD 103-HgR shows promise as a vector to deliver antigens and confer protection against AEEC pathogens (Keller et al., 2010).