• Vaccine Name: RCN-F1-V (Yersinia pestis F1 and V)
• Target Pathogen: Yersinia pestis
• Target Disease: Plague
• Vaccine Ontology ID: VO_0004692
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• Vector: raccoon poxvirus vaccine vector
  • Vector name: raccoon poxvirus vaccine vector
  • VO vector ID: VO_0001048
• Preparation: Recombinant raccoon poxvirus (RCN) expressing plague antigens (fraction 1 [F1] and a truncated form of the V protein-V307) (Rocke et al., 2010).
• Immunization Route: Intramuscular injection (i.m.)

Dog Response

• Host Strain: black-tailed prairie dogs (Cynomys ludovicianus)
• Vaccination Protocol: Voluntary consumption of baits expressing plague antigens several times over the course of several months to a group of 16 black-tailed prairie dogs. Another group of prairie dogs was injected subcutaneously (SC) (prime and boost) with 40 microg of F1-V fusion protein absorbed to alum, a vaccine-adjuvant combination demonstrated to elicit immunity to plague in mice and other mammals. Control animals received baits containing RCN without the inserted antigen or injected diluent (Rocke et al., 2010).
• Vaccine Immune Response Type: VO_0003057
• Challenge Protocol: Three weeks after consumption of the final vaccine-laden bait and 10 weeks after the injectable vaccine administration, animals were challenged with the CO92 wild-type isolate of Y. pestis (provided by USAMRIID), a highly virulent and well- characterized strain (Rocke et al., 2010).
• Efficacy: The oral vaccination of prairie dogs using RCN-based plague vaccines provided significant protection against challenge at dosages that simulate simultaneous delivery of the plague bacterium by numerous flea bites.