• Vaccine Name: Aeromonas hydrophila LSA34 vector vaccine WED/pUTDgap
• Target Pathogen: Aeromonas hydrophila
• Vaccine Ontology ID: VO_0004606
• Type: Recombinant vector vaccine
• Status: Research
• Antigen: The protective antigen GAPDH from the fish pathogen A. hydrophila, encoded by the gene gapA34 (Mu et al., 2011).
• Vector: Edwardsiella tarda EIB202 vaccine vector
  • Vector name: Edwardsiella tarda EIB202 vaccine vector
  • VO vector ID: VO_0001032
  • Description: Because E. tarda is commonly a fish pathogen, it is useful as a vector for other pathogens in fish. It has been shown to elicit strong immune responses (Mu et al., 2011).
• Preparation: The gapA34 gene was introduced into P(dps) and transformed into live, attenuated E. tarda strain EIB202 (Mu et al., 2011).
• Immunization Route: Intramuscular injection (i.m.)
• Description: A. hydrophila is a common fish pathogen, so in order to create a vaccine against this pathogen, the gapA34 gene of A. hydrophila was expressed in the bacterial vector E. tarda strain EIB202, and it was shown to increase survival of vaccinated fish (Mu et al., 2011).

Fish Response

• Host Strain: Turbot (Scophtalmus maximus)
• Vaccine Immune Response Type: VO_0000287
• Efficacy: Over 60% of the fish vaccinated with the WED/pUTDgap vector vaccine survived the A. hydrophila challenge (Mu et al., 2011).
• Description: The P(dps) promoter--which was chosen due to its high transcription activity-- was shown to elicit survival in turbot, meaning that it has the potential to be an important part of bacterial vaccine vector application (Mu et al., 2011) .