|
|
Vaccine Detail
|
P. yoelii DNA vaccine encoding MSP1 |
| Vaccine Information |
- Vaccine Name: P. yoelii DNA vaccine encoding MSP1
- Target Pathogen: Plasmodium spp.
- Target Disease: Malaria
- Vaccine Ontology ID: VO_0004593
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- MSP1 from P. yoelii str. 17XNL
gene engineering:
- Type: DNA vaccine construction
- Description:
- Detailed Gene Information: Click Here.
- DNA vaccine plasmid:
- DNA vaccine plasmid name:
- DNA vaccine plasmid VO ID: VO_0005072
- Immunization Route: Gene gun
|
| Host Response |
|
Mouse Response
- Vaccine Immune Response Type: VO_0003057
- Challenge Protocol: Two weeks after the final immunization, the mice were challenged i.p. with 10^5 P. yoelii pRBC. The course of infection was monitored by microscopic examination of tail-blood smears stained with Gimsa. (Sakai et al., 2003)
- Efficacy: MSP1 vaccine alone conferred partial protection. Vaccination with MSP1 + IL-12 conferred the strongest protective immunity against the infection. Only two of the six mice immunized with MSP1 alone survived, while five of the six mice immunized with MSP1 + IL-12 survived (Sakai et al., 2003).
|
| References |
Sakai et al., 2003: Sakai T, Hisaeda H, Nakano Y, Zhang M, Takashima M, Ishii K, Maekawa Y, Matsumoto S, Nitta Y, Miyazaki Ji, Yamamoto S, Himeno K. Gene gun-based co-immunization of merozoite surface protein-1 cDNA with IL-12 expression plasmid confers protection against lethal Plasmodium yoelii in A/J mice. Vaccine. 2003; 21(13-14); 1432-1444. [PubMed: 12615440].
|
|
