• Vaccine Name: Infectious Bronchitis Virus DNA Vaccine encoding S1, N, and M
• Target Pathogen: Infectious Bronchitis Virus (IBV)
• Target Disease: Infectious Bronchitis
• Vaccine Ontology ID: VO_0004584
• Type: DNA vaccine
• Status: Research
• N protein gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• S1 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• M membrane protein gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000024
• Immunization Route: Intramuscular injection (i.m.)

Chicken Response

• Vaccination Protocol: The seven day old SPF chickens were randomly divided into six groups with 20 chickens per group. Groups 1–3 received 100 micro-g of pVAX1-S1, PVAX1-M, or pVAX1-N,
  respectively; Group 4 received 100 micro-g combined DNA vaccine containing 33 micro-g of each of the three antigen plasmids; Groups 5–6 received 100 micro-g empty pVAX1 and 0.5 ml PBS. All the chickens were immunized intramuscularly with the vaccines at seven days of age (Yang et al., 2009).
• Vaccine Immune Response Type: VO_0003057
• Immune Response: Anti-IBV antibody levels were detected in chickens immunized with either pVAX1-S1, pVAX1-M, pVAX1-N or the three constructs in combination. In fact, a higher antibody titer level was observed in chickens immunized with the three expression constructs in combination compared with the individual constructs, suggesting a greater potency for inducing antibody response (Yang et al., 2009).
• Challenge Protocol: All chickens were challenged with 100EID50 of the IBV SAIBk strain in 0.1 ml by a nasal-ocular route at 21 days after the boost immunization (Yang et al., 2009).
• Efficacy: Immunization with the multivalent DNA vaccine provided up to 85% immune protection in the vaccinated chickens (Yang et al., 2009).