• Vaccine Name: Dengue Virus DNA Vaccine expressing prM/E proteins
• Target Pathogen: Dengue Virus
• Target Disease: Dengue Fever
• Vaccine Ontology ID: VO_0004547
• Type: DNA vaccine
• Status: Research
• Antigen: prM and E proteins from dengue virus 4 strain H241 (Lima et al., 2011)
• prM from Dengue virus 4 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• E from Dengue virus 4 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid: pCI DNA vaccine plasmid
  • DNA vaccine plasmid name: pCI DNA vaccine plasmid
  • DNA vaccine plasmid VO ID: VO_0000212
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: Ten 5-week-old female BALB/c mice per immunization group were inoculated three times into the quadriceps muscle with 100 μg of DENV-4-DNAv or pCI (empty vector), DENV-4 heat inactivated (1 × 10^5 PFU), or PBS. The mice were primed on day 0 and boosted 15 and 30 days after the initial inoculation (Lima et al., 2011).
• Vaccine Immune Response Type: VO_0003057
• Immune Response: The animals immunized with DENV-4-DNAv produced rising levels, after each vaccine inoculation, of specific neutralizing antibodies against dengue-4 virus (Lima et al., 2011).
• Challenge Protocol: Fifteen days after the third inoculation, the mice were challenged intracerebrally with a dose of 100LD50, prepared from a DENV-4-infected suckling mouse brain. Mouse mortality was monitored daily for 21 days (Lima et al., 2011).
• Efficacy: Immunization with DENV-4-DNAv induced significant protection against DENV-4 challenge, where 80% of the challenged mice survived (Lima et al., 2011).