• Vaccine Name: S. aureus DNA vaccine pClfaSrtD13
• Target Pathogen: Staphylococcus aureus
• Target Disease: Staph infections
• Vaccine Ontology ID: VO_0004541
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• Antigen: Clumping factor A (Clfa), fibronectin binding protein A (FnBPA) and the enzyme Sortase (Srt) from strains “Newman” or “8325-4” (Gaudreau et al., 2007)
• ClfA gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000212
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: All animals produced a mixed Th1 and Th2 response including functional antigen-specific, mostly IgG2a antibodies, sustained production of IFN-gamma and a predominantly CD8+ T-cell response (Gaudreau et al., 2007).
• Efficacy: Fourteen days after challenge, 70% of the vaccinated mice had survived as compared to only 15% of the control mice and after 21 days, the figures were 55% survival for the vaccinated as compared to 15% survival of the control group. However, signs of arthritis were observed in all the infected mice whether vaccinated or not (Gaudreau et al., 2007).