• Vaccine Name: T. gondii DNA vaccine pVAX-TgMIC13
• Target Pathogen: Toxoplasma gondii
• Target Disease: Toxoplasmosis
• Vaccine Ontology ID: VO_0004528
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• MIC13 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000024
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: Immunization with pVAX-TgMIC13 induced a strong immune responses demonstrated by significant lymphocyte proliferation, cytokine production and antibody responses (Yuan et al., 2013).
• Challenge Protocol: Acute: Subgroup A were challenged intraperitoneally with 1 x 10^3 tachyzoites of virulent T. gondii (RH strain) 14 day after the final immunization. The time of the death was recorded.
  Chronic: Subgroup B were challenged intragastrically with 10 cysts of cyst T. gondii (PRU strain) 14 day after the final immunization. The cysts in their brain were counted 30 days post challenge. (Yuan et al., 2013)
• Efficacy: Immunized mice showed increased survival time (21.3±11.3 days) and reduced number of cysts in brain of mice (57.14%) after challenge with tachyzoites of the virulent T. gondii RH strain and cysts of the T. gondii PRU strain, respectively (Yuan et al., 2013).