• Vaccine Name: E. maxima DNA vaccine pcDNA-Gam56
• Target Pathogen: Eimeria maxima
• Target Disease: Coccidiosis
• Vaccine Ontology ID: VO_0004527
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Chickens
• gam56 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005077
• Immunization Route: Intramuscular injection (i.m.)

Chicken Response

• Vaccination Protocol: The DNA vaccine was injected intramuscularly to yellow feathered broilers of 1-week old at 3 dosages (25, 50, and 100 µg/chick). Injection was repeated once 1 week later (Xu et al., 2013).
• Vaccine Immune Response Type: VO_0000286
• Immune Response: Compared with control groups, the administration of pcDNA-Gam56 vaccine markedly increased the lymphocyte proliferation activity (P<0.05) at day 7 and 14 after the first immunization. The level of lymphocyte proliferation started to decrease on day 21 after the first immunization. A similar trend was seen in specific antibody levels (Xu et al., 2013).
• Challenge Protocol: One week after the second injection, birds were challenged orally with 5×104 sporulated oocysts of E. maxima, then weighed and killed at day 8 post challenge (Xu et al., 2013).
• Efficacy: Among the 3 pcDNA-Gam56 immunized groups, the median dosage group displayed the highest lymphocyte proliferation and antibody levels (P<0.05). The median dosage group had the greatest relative body weight gain (89.7%), and the greatest oocyst shedding reduction (53.7%) (Xu et al., 2013).