• Vaccine Name: Classical Swine Fever Virus DNA Vaccine pcDNA/E2 with TRIF
• Target Pathogen: Classical swine fever virus
• Target Disease: Classical swine fever, hog cholera
• Vaccine Ontology ID: VO_0004524
• Type: DNA vaccine
• Status: Research
• E2 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid: pcDNA3.1 DNA vaccine plasmid
  • DNA vaccine plasmid name: pcDNA3.1 DNA vaccine plasmid
  • DNA vaccine plasmid VO ID: VO_0000158
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: Co-delivery of the DNA vaccine pcDNA/E2 with the TRIF adjuvant enhanced the cellular, not humoral, immune responses induced by DNA vaccines in mice (Wan et al., 2010).
• Challenge Protocol: All immunized pigs were intramuscularly challenged 2 weeks after the last immunization with 10^5 TCID50 highly virulent CSFV Shimen strain (Wan et al., 2010).
• Efficacy: All pigs co-immunized with pcDNA/E2 + pRK-TRIF survived viral challenge., though two pigs in this groups had a slight fever but recovered with 1 or 3 days and the remaining pig in this group had no CSF signs (Wan et al., 2010).

Pig Response

• Vaccine Immune Response Type: VO_0003057
• Challenge Protocol: All immunized pigs were intramuscularly challenged 2 weeks after the last immunization with 105 TCID50 highly virulent CSFV Shimen strain (Wan et al., 2010).
• Efficacy: All pigs co-immunized with pcDNA/E2 + pRK-TRIF survived viral challenge., though two pigs in this groups had a slight fever but recovered with 1 or 3 days and the remaining pig in this group had no CSF signs (Wan et al., 2010).