• Vaccine Name: Herpes Simplex Virus 2 DNA Vaccine encoding gD with CD40L
• Target Pathogen: Herpes simplex virus type 1 and 2
• Target Disease: Herpes
• Vaccine Ontology ID: VO_0004523
• Type: DNA vaccine
• Status: Research
• gD gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000132
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: The quadriceps muscles of BALB/c mice were injected with 10–60 mg of pgD DNA constructs formulated in a final volume of 100 ml of phosphate-buffered saline and 0.25% bupi- vacaine-HCl, using a 28-gauge needle. Forty micrograms of CD40 and CD40L gene expression cassettes was mixed with pgD plasmid solution prior to injection (Sin et al., 2001).
• Vaccine Immune Response Type: VO_0000286
• Immune Response: When coinjected with CD40L plus pgD vaccines, a significant increase in Th cell-proliferative responses was observed. Specifically, CD40L drives immune responses toward a Th1 phenotype (Sin et al., 2001).
• Challenge Protocol: After DNA injection, mice were challenged intravaginally with HSV-2 strain 186 with some modification. Before inoculating the virus, the intravaginal area was
  swabbed with a cotton-tipped applicator soaked with 0.1 M NaOH solution and then cleared with dried cotton applicators (Sin et al., 2001).
• Efficacy: When mice were immunized with gD plasmid DNA and then challenged with 4 LD50, 60% survival of gD plasmid-vaccinated animals was noted. When covaccinated with CD40L, 100% of the challenged animals survived (Sin et al., 2001).