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Vaccine Detail
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PRRSV DNA vaccine pCA-U-optiGP5 |
| Vaccine Information |
- Vaccine Name: PRRSV DNA vaccine pCA-U-optiGP5
- Target Pathogen: Porcine respiratory and reproductive syndrome virus
- Target Disease: Porcine Reproductive and Respiratory Syndrome (PRRS), Blue-Ear Pig Disease
- Vaccine Ontology ID: VO_0004506
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Pig
- ORF5
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pCAGGS expressed the U-optiGP5 fusion gene was generated by linking optiGP5 downstream of ubiquitin. The Kozak sequence, GCCACC, served as the upstream start codon for all three fragments (Hou et al., 2008).
- Detailed Gene Information: Click Here.
- ubiquitin
gene engineering:
- Type: DNA vaccine construction
- Description: This vaccine expressed a sequence encoding pig ubiquitin that had its Gly76 changed to Ala76 (Hou et al., 2008).
- Detailed Gene Information: Click Here.
- DNA vaccine plasmid: Pcaggs DNA vaccine plasmid
- Immunization Route: Intramuscular injection (i.m.)
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| Host Response |
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Pig Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: Four of six pigs in the pCA-U-optiGP5 group were devoid of visible pathological changes that were present in other vaccinated and control animals after challenge (Hou et al., 2008).
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| References |
Hou et al., 2008: Hou YH, Chen J, Tong GZ, Tian ZJ, Zhou YJ, Li GX, Li X, Peng JM, An TQ, Yang HC. A recombinant plasmid co-expressing swine ubiquitin and the GP5 encoding-gene of porcine reproductive and respiratory syndrome virus induces protective immunity in piglets. Vaccine. 2008; 26(11); 1438-1449. [PubMed: 18262692].
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