• Vaccine Name: Vaccinia virus DNA vaccine tPA-D8L
• Target Pathogen: Vaccinia virus
• Vaccine Ontology ID: VO_0004487
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• D8L gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pVax1TM expressed the transmembrane (TM) region and its downstream cytoplasm tail were removed from the wild type D8L gene , and a human tissue plasminogen activator (tPA) leader sequence was added to the N terminus of D8L coding gene (Sakhatskyy et al., 2006).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005071
• Immunization Route: Gene gun

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: Individual outer membrane proteins from intracellular and extracellular virions of vaccinia virus, when delivered in the form of either DNA vaccines or recombinant protein vaccines produced from baculovirus-infected insect cells, were able to protect mice from the vaccinia virus challenge and rhesus macaques from the monkeypox virus challenge (Sakhatskyy et al., 2006).