• Vaccine Name: B. anthracis DNA vaccine pCPA
• Target Pathogen: Bacillus anthracis
• Target Disease: Anthrax
• Vaccine Ontology ID: VO_0004480
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• pagA gene engineering:
  • Type: DNA vaccine construction
  • Description: The gene fragment encoding amino acids 175 to 764 of a B. anthracis PA protein was PCR amplified using the forward primer 5′-ACA AGT CTC GAG ACC ATG GTT CCA GAC CGT GAC-3′ and the reverse primer 3′-CTC TAT CCT ATT CCA TTA AGA TCT ACT AAA-5′, with the pYS2 template. The PA gene fragment expressed corresponds to the biologically active, protease-cleaved PA63 fragment of the full-length 83-kDa protein. The PCR product was then digested with two restriction enzymes XhoI and XbaI and ligated into the eucaryotic expression plasmid pCI (Promega, Inc., Madison, Wis.) (Price et al., 2001).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000212
  • Description: The pCl was used for encoding a biologically active portion of B. anthracis PA (Price et al., 2001).
• Immunization Route: Gene gun

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: All mice immunized with pCLF4, pCPA, or the combination of both survived the challenge, whereas all unimmunized mice did not survive (Price et al., 2001).