• Vaccine Name: Melanoma DNA vaccine pN4a-MAGE-1-GM
• Target Pathogen: Cancer
• Target Disease: Cancer
• Vaccine Ontology ID: VO_0004435
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• GM-CSF (Mus musculus) gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pNGVL4a expressed GM-CSF and MAGE-1 (Sun et al., 2002).
  • Detailed Gene Information: Click Here.
• MAGEA1 (MAGE1) gene engineering:
  • Type: Recombinant protein preparation
  • Description: (Sun et al., 2002)
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005014
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: C57BL/6 mice were immunized on days 0, 7, and 17 with the different MAGE-1 plasmids. After 6 days, the mice were challenged with B16 melanoma cells that express the human MAGE-1 antigen. Mice immunized with plasmids co-expressing MAGE-1 and GM-CSF had significantly fewer tumor colonies on their lungs than mice from any of the other immunization groups. In fact, 60% of the mice immunized with DNA vaccines that co-express MAGE-1 and GM-CSF had fewer than five tumor colonies on their lungs, whereas this was true of a little less than 20% of the mice immunized with MAGE-1 alone vaccines or co-inoculation with MAGE-1 vaccines and GM-CSF expressing plasmid (Sun et al., 2002).