• Vaccine Name: Carcinoma DNA vaccine pVAX/E2A
• Target Pathogen: Cancer
• Target Disease: Cancer
• Vaccine Ontology ID: VO_0004426
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• E2A gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000019
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: Eighty percent of B cell competent μMT+/− mice immunized with pVAX/E2A and GM-CSF plasmids were protected from HER-2 expressing tumor challenge. All mice challenged with the HER-2 negative untransfected control line developed tumors. Of note, protection was equal or better in B cell deficient animals, as 90% of the μMT−/− mice that were immunized with pVAX/E2A and GM-CSF plasmids rejected the tumor. All mice immunized with pVAX/E2A without the GM-CSF plasmid developed tumors. Thus, this HER-2 specific resistance to tumor challenge is entirely dependent on co-administration of the GM-CSF plasmid. None of the μMT−/− mice immunized with pVAX/E2A or the combination of GM-CSF and pVAX/E2A were protected from challenge with the HER-2 negative D2F2 control line (Lindencrona et al., 2004).