• Vaccine Name: West Nile virus DNA vaccine pCBWN encoding prM and E
• Target Pathogen: West Nile virus
• Target Disease: West Nile fever
• Vaccine Ontology ID: VO_0004415
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse, horse
• E protein gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pCBINT expressed WNV E protein (Davis et al., 2001).
  • Detailed Gene Information: Click Here.
• PrM gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pCBINT expressed WNV prM protein (Davis et al., 2001).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000116
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: Recombinant plasmid-transformed COS-1 cells expressed and secreted high levels of WN virus prM and E proteins into the culture medium (Davis et al., 2001).
• Efficacy: The lowest dose tested (0.1 μg of DNA per animal) is sufficient to induce complete protective immunity by our vaccine in mice. It was evident that the presence of WN virus antibody correlated with protective immunity, since all mice immunized with WN virus DNA remained healthy after virus challenge. All control mice developed symptoms of central nervous system infection 4 to 6 days later and died an average of 6.9 and 7.4 days after i.p. or infected-mosquito challenge, respectively (Davis et al., 2001).