• Vaccine Name: Autoimmune diabetes DNA vaccine pCMV–1/rGAD65 encoding GAD
• Target Pathogen: Diabetes
• Target Disease: Diabetes
• Vaccine Ontology ID: VO_0004404
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• gad65 gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pCMV expressed glutamic acid decarboxylase (GAD), an initial target islet antigen of autoimmune T cell repertoire (Balasa et al., 2001).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid: pCMV-1 DNA vaccine plasmid
  • DNA vaccine plasmid name: pCMV-1 DNA vaccine plasmid
  • DNA vaccine plasmid VO ID: VO_0000216
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0003057
• Efficacy: During the 25- week period of monitoring, the GAD65-plasmid DNA vaccination effectively prevented IDDM in young female NOD mice (1/13; 8% incidence). We also immunized mice with GM-CSF encoding GAD65 bicistronic vector (since it is known to recruit antigen-presenting cells, inducing a stronger immune response). However, GAD65–GM-CSF-plasmid DNA immunization (1/8; 12.5% incidence) did not alter the incidence of diabetes (P = 0.6477 vs GAD65-plasmid DNA). Female NOD mice in the control group readily became diabetic beginning at 17 weeks of age (3/12; 25% incidence) and reached a 75% incidence (9/12) at 23 weeks of age (P = 0.001 vs GAD65-plasmid DNA; P = 0.006 vs GAD65– GM-CSF-plasmid DNA) (Balasa et al., 2001).