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Vaccine Detail
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EAU DNA vaccine IRBP-DNA encoding IRBP |
| Vaccine Information |
- Vaccine Name: EAU DNA vaccine IRBP-DNA encoding IRBP
- Target Pathogen: Experimental autoimmune uveitis
- Target Disease: Experimental autoimmune uveitis
- Vaccine Ontology ID: VO_0004402
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- IRBP from humans
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pcDNA3.1 expressed the uveitogenic retinal Ag interphotoreceptor retinoid-binding protein (IRBP) (Silver et al., 2007).
- Detailed Gene Information: Click Here.
- DNA vaccine plasmid:
- DNA vaccine plasmid name:
- DNA vaccine plasmid VO ID: VO_0000158
- Immunization Route: Intravenous injection (i.v.)
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| Host Response |
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Mouse Response
- Vaccine Immune Response Type: VO_0000286
- Immune Response: IRBP-specific CD4+CD25high T cells could be cultured from vaccinated mice and transferred protection to unvaccinated, EAU-challenged recipients. Expression of IRBP in the periphery by DNA vaccination results in tolerance that acts at least in part through induction of IRBP-specific, FoxP3+CD4+CD25+ regulatory T cells (Silver et al., 2007).
- Efficacy: Vaccinated mice were highly protected from EAU induced by immunization with IRBP for at least 10 weeks after vaccination (Silver et al., 2007).
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| References |
Silver et al., 2007: Silver PB, Agarwal RK, Su SB, Suffia I, Grajewski RS, Luger D, Chan CC, Mahdi RM, Nickerson JM, Caspi RR. Hydrodynamic vaccination with DNA encoding an immunologically privileged retinal antigen protects from autoimmunity through induction of regulatory T cells. Journal of immunology (Baltimore, Md. : 1950). 2007; 179(8); 5146-5158. [PubMed: 17911600].
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