• Vaccine Name: Hepatitis B DNA vaccine pCMV-HBs encoding HBsAg
• Target Pathogen: Hepatitis B virus
• Target Disease: Hepatitis B
• Vaccine Ontology ID: VO_0004358
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• HbS gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pCMV expressed HBV surface antigen (HBsAg) (Davis et al., 1993).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000215
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: The serum concentration of secreted HBsAg after a one-time injection of DNA was sufficient to induce the production of anti-HBsAg 10 days after injection, and the antibody levels continued to increase for up to at least 60 days. Direct intramuscular injection of the plasmid vector encoding the HBsAg leads to secretion of the viral surface protein into the circulation, in the form of empty particles (Davis et al., 1993).
• Efficacy: A level of 10 mlU/ml of anti-HBsAg antibody is recognized as being sufficient in humans to confer protection against natural Hepatitis B virus infection. This level of antibody response was achieved in 68% of mice vaccinated with the vaccine candidate at two weeks after vaccination. By 8 wks, all mice had >100 mIU anti-HBsAg in their sera, suggesting sufficient vaccine efficacy (Davis et al., 1993).