• Vaccine Name: Hepatitis B DNA vaccine pS encoding major envelope proteins
• Target Pathogen: Hepatitis B virus
• Target Disease: Hepatitis B
• Vaccine Ontology ID: VO_0004357
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• Envelope proteins gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3 expressed HBV major envelope proteins (Chow et al., 1998).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000132
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: Coexpression of IL-2 and hepatitis B virus HBV^3 envelope protein within the same plasmid vector resulted in a dramatic increase in its ability to induce humoral and cellular immune responses to HBsAg. Also, the IL-2 adjuvant activity helps the HBV DNA vaccine elicit high anti-HBs titers in animals that usually fail to respond to rHBsAg vaccination (Chow et al., 1998)
• Efficacy: Four of five mice immunized with pS + pcDNA3 and challenged with CT26/S showed an inhibition of tumor growth. The protective efficacy was dramatically increased when the IL-12 gene was coinjected with plasmid pS because tumor growth was significantly suppressed, and two of five mice remained tumor free up to 60 days following tumor challenge (Chow et al., 1998).