• Vaccine Name: Influenza virus DNA vaccine NP DNA
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004308
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• NP from Influenza A virus (A/Puerto Rico/8/34(H1N1)) gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector V1J expressed the NP gene from influenza virus A/PR/8/34 (H1N1) (Fu et al., 1999).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000328
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: To assess the effectiveness of NP DNA immunization in comparison with influenza virus infection in the induction of CTL responses, we monitored the frequency of CTL precursors (CTLp) in mice following i.m. injection with NP DNA. We observed a positive correlation between the frequency of NP147-155 epitope-specific CTLp and the extent of protective immunity against cross-strain influenza challenge induced by NP DNA injection. In these studies immunization with NP DNA conferred protection against a lethal dose challenge with the influenza virus A/HK/68 (H3N2), a virus strain that conserves the sequence encoding NP including the H-2Kd-restricted CTL epitope 147–155, but contains significant changes in the viral surface glycoproteins (hemagglutinin (HA) and neuraminidase) compared with A/PR/8/34 (H1N1) (Fu et al., 1999).