• Vaccine Name: Influenza virus DNA vaccine d1TTFC-NP encoding NP
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004306
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• NP gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3.1 expressed influenza A nucleonucleoprotein epitope (amino acids 366–374; NP366) fused to the carboxy terminus of a tetanus toxin fragment (d1TTFC-NP) (Bins et al., 2005).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000158
• Immunization Route: Intradermal injection (i.d.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: This vaccination strategy confers protecting humoral immunity against influenza A infection within 2 weeks after the start of vaccination. In the week after infection, mice that had previously been exposed to influenza A virus showed a minimal weight loss (1% at day 4 after infection). In contrast, mice given tattoo vaccination with the control plasmid or given an intramuscular injection of the hemagglutinin construct showed a sizable (10% and 14%, respectively) drop in body weight. Notably, mice that had received a short-interval hemagglutinin tattoo were largely protected from influenza A−induced morbidity (maximal weight loss of 4%; Student t-test, P = 0.039 versus control vaccine). The protection correlated with the induction of neutralizing antibodies. Furthermore, intradermal DNA tattooing also conferred long-term (4 months) protection (Bins et al., 2005).