• Vaccine Name: Escherichia coli rfaL mutant vaccine
• Target Pathogen: Escherichia coli
• Target Disease: Hemorrhagic colitis
• Vaccine Ontology ID: VO_0002839
• Type: Live, attenuated vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• rfaL gene engineering:
  • Type: Gene mutation
  • Description: This mutant is from Escherichia coli (Billips et al., 2009).
  • Detailed Gene Information: Click Here.
• Immunization Route: Bladder instillation

Mouse Response

• Persistence: An rfaL mutant is attenuated in mice (Billips et al., 2009).
• Efficacy: An rfaL mutant induces significant protection in mice from challenge with wild type E. coli. Protection lasted more than 8 weeks (Billips et al., 2009).
• Host IL-6 response
  • Description: NU14 ΔwaaL significantly enhanced mouse macrophage IL-6 secretion relative to wild-type NU14 4 hours after treatment. TNF-alpha reduced IL-6 expression of deletion mutants, but under these conditions IL-6 was expressed more than that expressed by macrophages infected with the wild type in the presence of TNF-alpha (Billips et al., 2009).
  • Detailed Gene Information: Click Here.