• Vaccine Name: Staphylococcus aureus aroA mutant vaccine
• Target Pathogen: Staphylococcus aureus
• Target Disease: Staph infections
• Vaccine Ontology ID: VO_0002797
• Type: Live, attenuated vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• aroA gene engineering:
  • Type: Gene mutation
  • Description: This aroA mutant is from Staphylococcus aureus (Buzzola et al., 2006).
  • Detailed Gene Information: Click Here.
• Immunization Route: Intramammary immunization

Mouse Response

• Persistence: An aroA mutant is attenuated in mice (Buzzola et al., 2006).
• Efficacy: An aroA mutant induces significant protection in mice from challenge with wild type S. aureus (Buzzola et al., 2006).
• Host Ifng (Interferon gamma) response
  • Description: Ninety-six hours after challenge with the wild-type strain RN6390 or heterologous strain MB319, the relative mRNA levels of IFN-γ and IL-4 were determined in mammary glands by RT-PCR. Mammary glands from mice immunized with the aroA mutant showed a significant increase in the level of IFN-γ transcripts compared with control, unvaccinated mice (Buzzola et al., 2006).
  • Detailed Gene Information: Click Here.
• Host Il4 (interleukin 4) response
  • Description: Ninety-six hours after challenge with the wild-type strain RN6390 or heterologous strain MB319, the relative mRNA levels of IFN-γ and IL-4 were determined in mammary glands by RT-PCR. Mice immunized with the aroA mutant showed a significant increase in the level of IL-4 transcripts compared with control, unvaccinated mice (Buzzola et al., 2006).
  • Detailed Gene Information: Click Here.