• Vaccine Name: Yersinia pestis nlpD mutant vaccine
• Target Pathogen: Yersinia pestis
• Target Disease: Plague
• Vaccine Ontology ID: VO_0002942
• Type: Live, attenuated vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• nlpD gene engineering:
  • Type: Gene mutation
  • Description: This nlpD mutant is from Yersinia pestis (Tidhar et al., 2009).
  • Detailed Gene Information: Click Here.
• Immunization Route: subcutaneous injection

Mouse Response

• Persistence: An nlpD mutant is highly attenuated in mice (Tidhar et al., 2009).
• Efficacy: An nlpD mutant induced significant protection from challenge with wild type Y. pestis in mice (Tidhar et al., 2009).
• Host TNF-alpha response
  • Description: The nlpD-null mutant preserves the ability to translocate Yop effectors into host cells as evidenced by suppression of TNF-α secretion from infected RAW264.7 macrophages as compared to other mutant strains. TNF-alpha levels were similar to the levels found in wild type infection, which was significantly less than Yop mutants (Tidhar et al., 2009).
  • Detailed Gene Information: Click Here.