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Vaccine Detail
MDA-modified human apo B-100 peptide Vaccine |
Vaccine Information |
- Vaccine Name: MDA-modified human apo B-100 peptide Vaccine
- Target Pathogen: Atherosclerosis
- Target Disease: Atherosclerosis, arteriosclerotic vascular disease (ASVD)
- Vaccine Ontology ID: VO_0004276
- Type: Subunit vaccine
- Status: Research
- Antigen: MDA-modified apo B-100 (Fredrikson et al., 2005).
- Adjuvant:
- Immunization Route: Intramuscular injection (i.m.)
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Host Response |
Mouse Response
- Host Strain: Male apo E0 mice on C57BL/6 background
- Vaccination Protocol: Mice (n = 8–10 per group) were given a first injection with a MDA modified peptide conjugated to the carrier or the carrier alone at 6 weeks of age and booster injections 3 and 5 weeks later. Each injection contained 50 mg of the MDA-modified peptide conjugated to 50mg of the carrier cBSA (cationized bovine serum albumin) dissolved in 0.083M sodium phosphate 0.9M NaCl pH 7.2, according to the manufacture’s protocol (No. 77652, Pierce, Rockford, IL, USA), and with Alum (aluminum hydroxide, Pierce) included in all injections as adjuvant. The mice were fed a cholesterol diet (0.15% cholesterol, 21% fat; Lactamin AB, Kimstad, Sweden) from the age of 10 weeks (Fredrikson et al., 2005).
- Efficacy: Immunization with fragment P45 reduced atherosclerosis with 48%; fragment P74with 31; whereas immunization with P240 had no effect (Fredrikson et al., 2005).
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References |
Fredrikson et al., 2005: Fredrikson GN, Andersson L, Söderberg I, Dimayuga P, Chyu KY, Shah PK, Nilsson J. Atheroprotective immunization with MDA-modified apo B-100 peptide sequences is associated with activation of Th2 specific antibody expression. Autoimmunity. 2005; 38(2); 171-179. [PubMed: 16040338].
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