• Vaccine Name: E. coli CS3 in PLGA microspheres
• Target Pathogen: Escherichia coli
• Target Disease: Hemorrhagic colitis
• Vaccine Ontology ID: VO_0004262
• Type: Subunit vaccine
• Status: Research
• Antigen: CS3 colonization factor isolated from enterotoxigenic Escherichia coli (ETEC) (Byrd and Cassels, 2006).
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001341
  • Description: PLGA microspheres
• Immunization Route: intranasal immunization

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Four micrograms of CS3, 4 µg CS3 plus 2 µg mLT or 0·364 mg solid CS3-encapsulated PLGA microspheres (4 µg CS3 protein) were administered in a 10 µl volume drop-wise to the external nares of each mouse using a 2–20 µl Pipetteman (Ranin Instrument). Immediately prior to immunization, the vaccines were diluted with PBS to a concentration of 4 µg CS3 protein 10 µl–1. Control mice were likewise administered 10 µl PBS (Byrd and Cassels, 2006).
• Immune Response: the CS3-loaded PLGA microspheres induced significantly greater (P<0.001) serum and mucosal antibody responses than native CS3 (Byrd and Cassels, 2006).