• Vaccine Name: Recombinant PA with Poly(I:C) Adjuvant
• Target Pathogen: Bacillus anthracis
• Target Disease: Anthrax
• Vaccine Ontology ID: VO_0004255
• Type: Subunit vaccine
• Status: Research
• Antigen: Recombinant PA (Sloat and Cui, 2006).
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001332
• Immunization Route: intranasal immunization

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Mice were lightly anesthetized and given a total volume of 20 mL of rPA/pI:C solution in two 10-mL doses, with 10-15 min between each dose, half in each nare. As controls, mice (n = 5) were subcutaneously (s.c.) injected with rPA (5 mg/mouse) admixed with aluminum hydroxide gel, nasally dosed with rPA admixed with cholera toxin as a mucosal adjuvant, or left untreated. Mice were dosed on days 0, 7, and 14 (Sloat and Cui, 2006).
• Immune Response: Mice nasally immunized with rPA adjuvanted with pI:C developed strong systemic and mucosal anti-PA responses with lethal toxin neutralization activity. These immune responses compared favorably to that induced by nasal immunization with rPA adjuvanted with cholera toxin. Poly(I:C) enhanced the proportion of DCs in local draining lymph nodes and stimulated DC maturation (Sloat and Cui, 2006).