• Vaccine Name: Rotavirus Chimeric VP6 protein Vaccine
• Target Pathogen: Rotavirus
• Target Disease: Gastroenteritis
• Vaccine Ontology ID: VO_0004224
• Type: Subunit vaccine
• Status: Research
• Antigen: Chimeric VP6 protein (Choi et al., 2002).
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000351
  • Description: Adjumer
• Immunization Route: intranasal immunization

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Mice were lightly anaesthetized in a closed vessel with Metafane (methoxyflurane, Pitman–Moore Inc., Mundelein, IL). Vaccine formulations containing chimeric VP6 admixed with different adjuvants were then delivered intranasally or orally into groups of mice. Two i.n. doses (60 μl per dose, 30 μl per nostril) or two oral doses (100 μl per dose), separated by 2 weeks, were delivered. Each dose of immunogen consisted of 9 μg of purified chimeric VP6, which was mixed with LT(R192G) (10 μg), CTA1-DD (10 μg), Adjumer® (50 μg), CpG ODN (10 μg) or QS-21 (20 μg) just before immunization. The amounts of adjuvants used were based on the amounts determined for other immunogens. A group of unimmunized mice served as the control in each experiment (Choi et al., 2002).
• Immune Response: Mice immunized with Adjumer®, CTA1-DD or LT(R192G), also developed low titers of fecal rotavirus IgA. Use of Adjumer as an adjuvant had a large IgG1 titer but no increase in IgG2a titer (Choi et al., 2002).
• Challenge Protocol: Four weeks after the last immunization, mice were orally (gavage) challenged with 4×10^4 FFUs (100 μl, 100 SD50) of passage-9 EDIM rotavirus (Choi et al., 2002).
• Efficacy: Mice immunized with chimeric VP6 and Adjumer had a 35% reduction in shedding of virus compared to unimmunized mice (Choi et al., 2002).