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Vaccine Detail
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Brucella melitensis and Brucella abortus virB10 Omp31 Omp16 recombinant vector vaccine |
| Vaccine Information |
- Vaccine Name: Brucella melitensis and Brucella abortus virB10 Omp31 Omp16 recombinant vector vaccine
- Target Pathogen: Brucella spp.
- Target Disease: Brucellosis
- Vaccine Ontology ID: VO_0009620
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Cattle
- Host Species as Laboratory Animal Model: Escherichia coli SHuffle; (in silico models for docking and simulation)
- Antigen: virB10; Omp31; Omp16; 50S L7/L12 Ribosomal protein from Mycobacterium tuberculosis
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Adjuvant:
50S L7/L12 Ribosomal protein from Mycobacterium tuberculosis (bovine TLR4 and TLR9 agonist)
- Preparation: The multi-epitope construct was linked with GPGPG, AAY, and KK linkers, cloned into the pET-32a shuttle vector using BamHI and HindIII restriction sites, and expressed as a soluble ∼82 kDa protein in Escherichia coli SHuffle host. (Rahimnahal et al, 2023)
- Description: The study designed a multi-epitope vaccine targeting B. melitensis and B. abortus using virB10, Omp31, and Omp16 antigens, incorporating predicted T lymphocyte, cytotoxic T cell, and IFN-γ epitopes. The 50S L7/L12 ribosomal protein from Mycobacterium tuberculosis was included as a bovine TLR4 and TLR9 agonist to enhance immune stimulation. Efficacy: The construct showed stable interaction with bovine TLR4 and TLR9 receptors, suggesting potential to induce immune responses. No in vivo efficacy data reported. Immune response: The vaccine is predicted to stimulate T- and B-cell responses by presenting multiple epitopes and activating innate immunity via TLR4 and TLR9 agonism, potentially inducing IFN-γ production. (Rahimnahal et al, 2023)
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| Host Response |
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| References |
Rahimnahal et al, 2023: Rahimnahal S, Yousefizadeh S, Mohammadi Y. Novel multi-epitope vaccine against bovine brucellosis: approach from immunoinformatics to expression. Journal of biomolecular structure & dynamics. 2023; 41(24); 15460-15484. [PubMed: 36927475].
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