• Vaccine Name: Brucella suis wild type outer membrane vesicle subunit vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009571
• Type: Subunit vaccine
• Status: Research
• Host Species for Licensed Use: Pig
• Host Species as Laboratory Animal Model: Mus musculus
• Antigen: outer membrane vesicles
• Preparation: OMVs were isolated from wild-type B. suis using ultracentrifugation and characterized by electron microscopy, dynamic light scattering, SDS-PAGE, and proteomics; no adjuvant was reported. (González et al, 2024)
• Description: Outer membrane vesicles (OMVs) from wild-type B. suis 1330 strain were characterized and evaluated as an acellular vaccine candidate against systemic and mucosal B. suis infection. Efficacy: Immunization induced antigen-specific IgG and functional antibodies that reduced bacterial adherence and invasion and enhanced phagocytosis; conferred protection against intratracheal challenge but was less protective than ΔmapB OMVs against intraperitoneal infection. Immune response: Induced humoral immunity with antigen-specific IgG and functional antibodies; cellular immunity showed lower levels of interleukin-17 and gamma interferon compared to ΔmapB OMVs, indicating a less robust T helper 1 response. (González et al, 2024)

Mouse Response

• Immune Response: Induced humoral immunity with antigen-specific IgG and functional antibodies; cellular immunity showed lower levels of interleukin-17 and gamma interferon compared to ΔmapB OMVs, indicating a less robust T helper 1 response. (González et al, 2024)
• Challenge Protocol: Mice were intramuscularly immunized with wild-type OMVs; antigen-specific antibody levels and functional assays (inhibition of bacterial adherence/invasion, enhancement of phagocytosis) were performed; cellular immunity was assessed by cytokine secretion after spleen cell stimulation; protection was evaluated by challenging mice intraperitoneally or intratracheally with virulent B. suis and quantifying bacterial load. (González et al, 2024)
• Efficacy: Immunization induced antigen-specific IgG and functional antibodies that reduced bacterial adherence and invasion and enhanced phagocytosis; conferred protection against intratracheal challenge but was less protective than ΔmapB OMVs against intraperitoneal infection. (González et al, 2024)
• Description: Mice were intramuscularly immunized with wild-type OMVs; antigen-specific antibody levels and functional assays (inhibition of bacterial adherence/invasion, enhancement of phagocytosis) were performed; cellular immunity was assessed by cytokine secretion after spleen cell stimulation; protection was evaluated by challenging mice intraperitoneally or intratracheally with virulent B. suis and quantifying bacterial load. (González et al, 2024)