• Vaccine Name: live attenuated Brucella abortus strain 19 wbkA gene deletion vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009454
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Host Species as Laboratory Animal Model: Mus musculus (BALB/c mice)
• Antigen: wbkA gene deletion
• Preparation: A wbkA gene knockout mutant of Brucella abortus strain 19 was constructed to produce a strain deficient in LPS O-chain structure. (Nayeri et al, 2019)
• Description: The study aimed to create a gene knockout mutant of Brucella abortus strain 19 by deleting the wbkA gene, which encodes part of the LPS O-chain, to reduce diagnostic interference and maintain attenuation. Efficacy: The wbkA mutant induced a splenocyte proliferative response in mice comparable or superior to parental S19 and RB51 strains, indicating immunogenicity. Immune response: The mutant strain did not induce IgM antibody response detectable by ELISA, suggesting reduced interference in serological differentiation between vaccinated and infected animals. (Nayeri et al, 2019)

Mouse Response

• Host Strain: BALB/c
• Immune Response: The mutant strain did not induce IgM antibody response detectable by ELISA, suggesting reduced interference in serological differentiation between vaccinated and infected animals. (Nayeri et al, 2019)
• Efficacy: The wbkA mutant induced a splenocyte proliferative response in mice comparable or superior to parental S19 and RB51 strains, indicating immunogenicity. (Nayeri et al, 2019)
• Description: The mutant strain was tested in BALB/c mice to analyze splenocyte proliferative response and IgM antibody production compared to parental S19 and RB51 strains. (Nayeri et al, 2019)