• Vaccine Name: live attenuated Brucella ovis wbkC deletion wbdR tagged vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009439
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Sheep
• Host Species as Laboratory Animal Model: Mus musculus
• Antigen: wbkC deletion; wbdR expression
• Preparation: Live attenuated Brucella melitensis Rev. 1 strain with wbkC gene deletion and wbdR gene insertion. (Aragón-Aranda et al, 2019)
• Description: The vaccine is a modified Brucella melitensis Rev. 1 strain deleted in wbkC (lipopolysaccharide formyl-transferase) and carrying wbdR (E. coli acetyl-transferase) to induce an antibody response distinguishable from wild-type strains and to avoid interference with B. melitensis serodiagnosis. Efficacy: The modified Rev. 1 vaccine was comparatively attenuated and provided protection against Brucella ovis infection in mice. Immune response: The vaccine triggered antibodies that could be differentiated from those evoked by wild-type strains, avoiding interference with serodiagnosis of B. melitensis. (Aragón-Aranda et al, 2019)

Mouse Response

• Immune Response: The vaccine triggered antibodies that could be differentiated from those evoked by wild-type strains, avoiding interference with serodiagnosis of B. melitensis. (Aragón-Aranda et al, 2019)
• Challenge Protocol: Mouse model experiments assessing antibody response, attenuation, and protection against Brucella ovis challenge. (Aragón-Aranda et al, 2019)
• Efficacy: The modified Rev. 1 vaccine was comparatively attenuated and provided protection against Brucella ovis infection in mice. (Aragón-Aranda et al, 2019)
• Description: Mouse model experiments assessing antibody response, attenuation, and protection against Brucella ovis challenge. (Aragón-Aranda et al, 2019)